1. What isthe difference between an enzyme "cofactor" and anenzyme "coenzyme"?
2. What are the general properties of enzymes?
3. Define these: Transition state, reaction coordinate, ratedetermining step.
4. Draw and label a reaction coordinate diagram for anuncatalyzed reaction, S->P, and the same reaction catalyzed byan enzyme E.
5. Fifteen ug of an enzyme of Mr 30,000 working at Vmaxcatalyzes the conversion of 60 umol of substrate into product 3min. What is the enzyme's turnover number? Note: Mr is molecularweight.
6. For the reaction (below), the Michaelis-Menten constant, Km,is actually a summery of three terms. What are they? Explain whatthe terms mean? How is Km determinedgraphically?
7. A biochemist obtaines the following set of data for an enzymethat is known to follow Michaelis-Mentenkinetics.
.
1. What isthe difference between an enzyme "cofactor" and anenzyme "coenzyme"?
2. What are the general properties of enzymes?
3. Define these: Transition state, reaction coordinate, ratedetermining step.
4. Draw and label a reaction coordinate diagram for anuncatalyzed reaction, S->P, and the same reaction catalyzed byan enzyme E.
5. Fifteen ug of an enzyme of Mr 30,000 working at Vmaxcatalyzes the conversion of 60 umol of substrate into product 3min. What is the enzyme's turnover number? Note: Mr is molecularweight.
6. For the reaction (below), the Michaelis-Menten constant, Km,is actually a summery of three terms. What are they? Explain whatthe terms mean? How is Km determinedgraphically?
7. A biochemist obtaines the following set of data for an enzymethat is known to follow Michaelis-Mentenkinetics.
.