Question

We are using beer's law to calculate the molar extinction coefficient of a sample we collected through gel-filtration chromatography. The sample mixture that we put into our chromatography was .5ml of blue dextran, K2crO4, and myoglobin. The blue dextran eluted first and we ran the collection in the spectrophotometer and got our absorbance and path length values. I'm confused on how to calculate my concentration values. It says, "In your original sample,350 microliters of 1 mg/ml blue dextran was added to teh mixtyre. Assume you collect all of hte blue dextran after gel filtration column." My volume of collected blue dextran is 1ml.