Aspartame is an artificial sweetener that is 160 times sweeter than sucrose (table sugar) when dissolved in water. Is it marketed as NutraSweet. The molecular formula of aspartame is C₁₄H₁₈N₂O₅. What is the molar mass of aspartame?

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Christian TusoLv10

1 Dec 2020

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12. Analytical chem question on sapling

correct answer will get rated! Thanks

A student researcher performed a chromatographic separation of caffeine and aspartame with methanol as the mobile phase using a C-18 column. The retention times for methanol (tm), caffeine (tc), and aspartame (ta) were 40.0 s, 108.7 s, and 177.2 s, respectively.

A) What is the selectivity factor (relative retention), Î±, for this column?

B) Calculate the hypothetical retention times for caffeine and aspartame if their retention factors were reduced by 27.0%. Assume the mobile phase retention time (tm = 40.0 s) remains constant.

t caffeine = ? seconds; t aspartame = ? seconds

C) Choose the statements that correctly describe the outcome if the partition coefficient for caffeine (Kc) were to decrease (Hint: there is more than one correct answer).

1 The retention time of caffeine would increase. 2 The retention factor of caffeine would increase. 3 Caffeine would spend a greater amount of time in the mobile phase. 4 The retention time and retention factor of caffeine would not change. 5 The retention factor of caffeine would decrease. 6 The retention time of caffeine would decrease.

HINTS= The selectivity factor, or relative retention, for the column is the ratio of the retention factors of caffeine and aspartame. The retention factor is the ratio of the time a solute spends in the stationary phase to the time it spends in the mobile phase.

= The partition coefficient (Kc) describes the equilibrium of caffeine molecules between the stationary and mobile phases.