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Indicate which of the following factors can increase or decrease column efficiency in liquid chromatography. Give reasons in each case.
- Very low flow rate
- Large stationary phase particle size
- Small stationary phase particle size
- Regularly shaped stationary phase
- Irregularly shaped stationary phase
- High temperature
- Low temperature
- Non‐uniform stationary phase particle size
- High diffusion coefficient in mobile phase
- Low diffusion coefficient in mobile phase
- Longer column
- Large dead volume
Indicate which of the following factors can increase or decrease column efficiency in liquid chromatography. Give reasons in each case.
- Very low flow rate
- Large stationary phase particle size
- Small stationary phase particle size
- Regularly shaped stationary phase
- Irregularly shaped stationary phase
- High temperature
- Low temperature
- Non‐uniform stationary phase particle size
- High diffusion coefficient in mobile phase
- Low diffusion coefficient in mobile phase
- Longer column
- Large dead volume
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