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Name one stain to visualize proteins on a membrane AFTER you have transferred them from gel to membrane and how would you remove the stain to continue on to immunoblotting?

Please give a detailed explanation

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Elin Hessel
Elin HesselLv2
18 Jan 2019
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Related questions

1. What is the size of the fragment that contains the Alu insertion? Without the Alu insertion?

2. Describe the two functions of loading dye.

3. What is the purpose of using a marker or ladder during electrophoresis?

4. Suppose you have 4 fragments of 500bp each. How many bands would appear on the gel?

5. Describe the quick stain protocol that you will be following to visualize the DNA.

6. Suppose you run a gel, and you are concerned that you may have ‘lost’ some of the smallest fragments due to them running off the gel. What two things can change in ensure that these would remain on the gel the next time you repeat the experiment?

HELP WITH BIO MULTI CHOICE QUESTIONS:

QUESTION 2

Which of the following structures discussed in class is NOT found on all bacteria?
Flagella
Capsid
Fimbria
Plasma membrane


QUESTION 3
You are presented with a strain of bacteria that is resistant to the antibiotic penicillin. What steps would you take to confirm that you have a resistant bacterium?
Perform a Gram stain to determine pink stained Gram-positive bacteria.
Perform a Gram stain to determine purple stained Gram-positive bacteria.
Perform a Gram stain to determine pink stained Gram-negative bacteria.
Perform a Gram stain to determine purple stained Gram-negative bacteria.

QUESTION 4
With respect to bacterial growth into a fresh culture tube containing nutrient broth, at which phase do organisms undergo binary fission the most rapidly?
Lag phase
Stationary
Exponential
death

QUESTION 5
How would antibiotic resistance be passed from one bacterium to another bacterium?
Through the flagella via a plasmid.
Through the sex pilus via chromosomal DNA.
Through the sex pilus via a plasmid.
Through the flagella via chromosomal DNA.

QUESTION 6
When an F plasmid integrates into the host chromosome, the strain is referred to as

Hfr.
F'.
F-.
F+.

QUESTION 7
If you remove the peptidoglycan layer from a Gram-positive cell, it would still stain purple with a Gram stain (T/F).
True
False

QUESTION 8
During infection with a bacteriophage, bacterial DNA is packaged into a capsid and will:

be integrated into the genome of a new bacterium upon ligation.
be integrated into the genome of a new virion upon recombination.
be integrated into the genome of a new bacterium upon recombination.
be integrated into the genome of a new virion upon liagation.

QUESTION 9
Which of the following statements best describes most bacteria?
They are generally harmful.
They are limited to living in a few habitats.
They are very common in the environment.
They are responsible for the “common cold.”


QUESTION 10
Prokaryotes lack some parts found in eukaryotic cells, including which item?
a nuclear membrane
DNA
one or more chromosomes
a plasma membrane
all of the above

QUESTION 11
With respect to gram negative bacteria, what is the order of the bacterial cell structure from outside to inside?
Thin peptidoglycan layer, plasma membrane

thick peptidoglycan layer, plasma membrane

outer membrane, thick peptidoglycan layer, plasma membrane

outer membrane, thin peptidoglycan layer, plasma membrane


QUESTION 12
In transformation, a piece of DNA from a dead host can be taken up by living host

True
False

These Questions Are in regards to Stainingtechniques.

Endospores

1) Under what conditions are endospores usually formed?

2) How do Bacillus and Clostridium genera differ in their oxygenpreference?

3)After Staining, why do endospores appear green but the rest ofthe cell pink?

4) Name 3 diseases caused by anaerobicendospore forming organisms and one caused byaerobic endospore forming organisms.

Acid Fast Stain

1) The mycobacteria appear fuchsia with an Acid Fast Stain andpurple with a Gram Stain. Explain his phenomenon.

2) Why is it desireable to combine S. aureus with acid fastorganisms such as M. smegmatis when applying the Acid Fast stainingtechnique?

3) Name two human diseases and their causative agents where theAcid Fast stain is used in diagnosis.

Would greatly appreciate as detailed of an answer aspossible.

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