BIOL 200 Study Guide - Final Guide: Fluorescence Microscope, Signal Recognition Particle, Immunofluorescence
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Short answer: explain in one or 2 sentences why plants need to have mitochondria in every cell, even when the sun is shining, there are no motor proteins that move on intermediate filaments. Fill in the blanks: a small gtp-binding protein called. ___________ assembles around the neck of each coated vesicle and helps in pinching off the vesicle from the membrane: __________ is the motor protein that moves along cytoplasmic microtubules and towards the plus end of the microtubule. Design an experiment using appropriate experimental technique(s) to study the kinesin-assisted transport of macromolecules. Immunofluorescence microscopy: primary antibodies bind to cytoskeletal proteins. Secondary antibodies labeled with a fluorescent tag bind to the primary antibody. Fluorescence techniques: fluorescent versions of cytoskeletal proteins are made and introduced into living cells. Fluorescence microscopy and video cameras are used to view the proteins as they function in the cell.