BIOL 1111 Lecture Notes - Safranin, Signal Transduction, Attenuated Vaccine
1 Feb 2013
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Document Summary
Mutation: any inherited alteration that does not involve uptake of foreign genetic material. Allele: one of the different forms of a gene that can exist at a single locus. Wild type: what you would find in the wild. Phenotype: detectable outward manifestations of a specific genotype. Prototroph: wild type or revertants that do not require the added compound. Auxotroph: mutant that is deficient in the synthesis of an essential compound. Conditional mutation: mutant phenotype is observed only under certain circumstances. Permissive condition: environ conditions under which a conditional mutant shows the wt phenotype. Nonpermissive condition: doesn"t allow the protein to be normal, brings out the mutant phenotype. Selection: a protocol that allows you to eliminate by killing all of the non-mutant organisms and allows only the mutants to survive. Enrichment: selecting for auxotrophs by performing a screen in which you selectively kill of the normal cells/bacteria and eventually isolate the cells/bacs with the deleterious mutation.
