LIFESCI 2A03 Lecture Notes - Fibroblast, Cyclic Adenosine Monophosphate, Epithelium
2 Feb 2013
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Purpose: determining if msc can differentiate into aecs. Hypo: if created in a particular condition as described in method, the airway-epithelium culture condition may be useful to assay for differentiation of mscs to aecs. Fig ab&c shows the state of primary human aecs after being cultured for 2 weeks & how closely they mimic the morphology & function of surface epithelium conducting airways. Found: mscs can appear differently in different conditions: plastic surface normal condition gfp-mscs have slender fibro-blast like or flat polygonal cell shape, in mixed culture mscs assume epithelia like cuboidal or columnar cell shape. Purpose: determining whether the cocultured mscs had important epithelia-specific marker genes: ck-18 & occludin & 1st hypo: if it is cocultured with ck-18 antibody then mscs should differentiate into aecs. Result: only some (as seen in fig i-k) Fig a&b shows ck-18 effect on normal aecs. Fig cd, gh shows ck-18 present on gfp-mscs.
