BIO120H1 Lecture Notes - Lecture 11: Plasmid, Dna-Binding Domain, Tryptophan
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BIO120H1 Full Course Notes
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Let"s take stock: we"ve talked about the transcriptome, its involvement b/w organisms even within an organism. We"ve talked about transcription factor use, which gene regulatory proteins are used at which points in time & we"re going to determine ultimately what the transcriptome is that gets made in those cells. Proteins are functioning together in complexes that really drives differences b/w cells which is what we"re going to focus on in today"s lecture. Complete set of protein-protein interactions produced by the proteome at any one time. Helps us define, not just a few interactions b/w proteins but a good many interactions b/w proteins. Stan fields developed the yeast 2-hybrid assay. The yeast two-hybrid assay takes advantage of the way in which eucaryotic transcription factors/gene regulatory proteins work. Identify binding partners 2 proteins that interact with each other. Looking for the interaction b/w the bait protein & its corresponding. Gal4 has a dna-binding domain dbd and a transcription-activation domain.
