BIOL 2020 Study Guide - Final Guide: Okazaki Fragments, Urinary Tract Infection, Atp Hydrolysis
Document Summary
5: deoxynuclcleoside 5"-triphosphate, datp, dgtp, dctp, dttp as well as mg2+ and primer and proof reader. Free 3"-oh, bound to template strand, made of rna. Nucleophilic attack by the 3"-oh on the innermost phosphorous atom of the deoxynucleoside triphosphate, phosphodiester bond is created and releases pyrophosphate, this then yields 2 orthophosphates (by pyrophosphatase) occurs in the 5" 3" end. Polymerase , 3" 5" exonuclease ( high fidelity, less than 10^-8 error, from induced fit mechanism) and 5" 3" exonuclease site. Dna polymerase close down around (dntp), triggers conformational change (active site binding in polymerase), finger domain rotates to form tight pocket, base pair can now readily fit. Domain a1 and b1 of pcra bind single stranded dna, when atp binds the cleft between these 2 closes, domain a1 slides along dna, when atp is hydrolyzed the cleft opens, dna goes toward a1. Repeated over and over, and dna is unwound.
