CSB346H1 Lecture Notes - Viral Vector, Methysergide, Central Chemoreceptors
Document Summary
Csb346 tutorial assignment 2 notes (march 14, 2013) They injected a virus so they could transduce chr2. If the control existed, it would turn red, but they are not going to fire when you shine them with light. The viral construct transduce chr2 in the neurons so they can be activated by light: this is not the control. epet-cre. They expressed the viral vector in epet-cre mice. It is a specific transgenic mouse line where it has an epet-cre promoter region. This means that epet-cre (pet-1) is a transcription factor that allows specific targeting because this particular transcription factor is involved with the development of serotonergic neurons. Only serotonergic neurons have the epet-cre transcription factor. This is a transgenic mouse line that is expressing epet-cre. They selectively targeted serotonergic neurons by using epet-cre as the transgene because epet-cre is specific to serotonergic neurons. Cre-recombinase is the transgene that is only expressed in serotonergic neurons.
