BIOL308 Lecture Notes - Lecture 1: Replica Plating, Expression Vector, Flowering Plant
2 May 2017
School
Department
Course
Professor
Document Summary
1: alter its coding region through dna fragment insertion. 2: prevent it from being expressed at all morpholino antisense oligonucleotides to bind to mrna and block it from being transcribed. 3: prevent translation of a specific mrna to eliminate its function. Promoter exon 1 intron exon 2. 7 methyl guanine cap at 5" exon 1 exon 2 poly a tail at 3". Important to understand capping, splicing, ribozymes, post translational processing, processing of t/rrna. Compiling large amounts of molecular data (genomes, dna, rna, proteins) and allow comparison of new sequences to previous ones. Isolate temperature sensitive mutants in yeast by adding a mutagen (causes non-lethal dna changes) and plating and replicating the samples (replica plating) while testing for growth in various temperatures (where 23 is normal and 36 is not). If there is no growth at the higher temperature that colony contains yeast that is temperature sensitive. The gene should then be observed to find what causes temperature sensitivity.
