Biology 1002B Lecture Notes - Lecture 3: Antibody, Protein Structure, Photon
15 Apr 2013
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What controls amount of protein in a cell: transcription: copying of dna to mrna, can regulate the expression of specific genes that codes for specific protein. Transcript abundance, how much of the corresponding protein mrna in the cell. The mrna can break down, so it also depends on how long they can stay in contact, some might decay in minutes some in hours. Isolate total rna from cell or tissue samples: run the sample on a gel electrophoresis. Dominant rna in our cells is rrna (ribosomal rna) Mrna are very little compared to rrna transfer the electrophoresis onto a membrane, We use a probe: we expose the membrane to radioactive gene-specific probe . Probe is single stranded dna, it will hybridize with the mrna on the membrane. We made the probe, so we know the sequences, high efficiency, will bind with membrane mrna very effectively.
