BIOC13H3 Lecture Notes - Lecture 8: Peripheral Membrane Protein, Q Cycle, Atp Synthase
Document Summary
A, b or c based on absorption spectrum. Etc has a, a3, bl (b566), bh (b562), c1 are all integral proteins and cyt c is a peripheral membrane protein on the outer surface of imm. Each carry 1 e- per heme iron. Etc links redox energy to atp synthesis. Involves pumping out protons from the matrix to imm and protons flowing back through the. Atp synthase in response to the chemical and electrical differential. Unequal distribution of [h+] is energy rich called proton motive force. Proton circuit is established where protons respond to the chemical and electrical gradient and flow back to matrix to equalize distribution though atp synthase to catalyze atp synthesis. Mitochondria oxidizes substrates and consume oxygen only when adp is present. Mitchell"s proposal: synthetic compounds uncouple oxidation from phosphorylation (using synthetic molecules disrupts the atp synthesis) In bacteriorhodopsin, reconsitituion in membrane vesicles with atp synthase and a proton pump.
