BIOL308 Study Guide - Kozak Consensus Sequence, Ribosome-Binding Site, Eif2

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goldmink881

13 Jun 2013

School

University of Waterloo

Department

Biology

Course

BIOL308

Professor

Dragana Miskovic

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Document Summary

It"s a conserved sequence of nucleotides (aggaggu) 8-13 nt upstream from the first codom to be translated. It functions as a ribosomal binding site and binds the mrna to the ribosome (pairs with the sequence at the 3" end of the 16s rrna of the small. It"s a sequence that occurs on eukaryotic mrna (ccrccaugg; r=a or g). The sequence is recognized by the 40s ribosomal subunit (with trna already charged) of. On the other hand, prokaryotes add a formyl group (cho) to the methionine and therefore, carry fmet (formyl methionine) which is easily recognizable from other methionines: the 40s ribosomal subunit of ribosomes recognizes the kozak sequence in. The methyl cap interaction also plays a role in the binding. However, in prokaryotes, the shine-dalgarno sequence binds to the 30s ribosomal subunit. In eukary, trna forms ternary complex before binding to ribosome (in pro, just trna binds to p site).

Related Questions

What is the role of the following in translation: AUG codon, A site, P site, peptide bond, tRNA, rRNA, ribosome subunit, N terminus, C terminus, R group, initiation codon, fMet, Shine-Dalgarno, peptidyl transferase, release factor, nonsense codons, antibiotics.

goldgnu271

Please please please I reallly need help with the following 5 questions.

Question 1

Which of the following is responsible for directing the prokaryotic ribosome to the +1 start site:

	a.	the 3' UTR
	b.	the AUG codon
	c.	Kozak sequence
	d.	Okasaki fragment
	e.	Shine Dalgarno sequence

Question 2

In translation, mRNAs are READ in the:

	a.	5' to 3' direction and polypeptide chains are synthesized amino to the carboxyl ends
	b.	5' to 3' direction and polypeptide chains are synthesized carboxyl to the amino ends
	c.	3' to 5' direction and polypeptide chains are synthesized carboxyl to the amino ends
	d.	3' to 5' direction and polypeptide chains are synthesized amino to the carboxyl ends

Question 3

Which of the following terms describes a tRNA to which an amino acid has been added:

	a.	charged tRNA
	b.	activated tRNA
	c.	stimulated tRNA
	d.	hydrolyzed tRNA

Question 4

The ability of some tRNA to recognize more than one codon in mRNA is explained by which of the following:

	a.	a reading frameshift
	b.	a reading mistake
	c.	Redundancy Theory
	d.	Wobble Hypothesis

Question 5

One essential component in eukaryotic mRNAs recognized by translation initiation factors is the

	a.	TATA box
	b.	AUG initiation codon
	c.	7-methylguanosine cap
	d.	Shine-Dalgarno sequence

blackporcupine6

22. There are 20 amino acids and 64 possible codons. Which of the following is closer to the actual number of different tRNA molecules in a cell?

A. 4

B. 16

C. 20

D. 61

E. 64

23. There are 20 amino acids and 64 possible codons. How many different eukaryotic aminoacyltRNA synthetases are there? A. 1

B. 16

C. 20

D. 61

E. 64

24. Which way are the amino acid and tRNA attached in a charged tRNA?

A. Amino acid-5'tRNA3'-AMP B

. Amino acid-AMP-3'tRNA

C. AMP-amino acid-3'tRNA

D. Amino acid-3'tRNA5'-AMP

E. Amino acid-AMP-5'tRNA

25. The anticodon that recognizes the initiation codon in translation is

A. 5' AUG 3'

B. 3' AUG 5'

C. 5' ACU 3'

D. 5' UAC 3'

E. 3' UAC 5'

26. The first amino acid incorporated into a growing polypeptide

A. In bacteria is methionine and it is also methionine in eukaryotes

B. In bacteria is tryptophan, while it is methionine in eukaryotes

C. In bacteria is N-formyl methionine, while it is methionine in eukaryotes

D. In bacteria is N-formyl methionine and it is also N-formyl methionine in eukaryotes

E. In bacteria is methionine, while it is N-formyl methionine in eukaryotes

27. In E. coli the IF3 forces tRNAf Met to bind with

A. The A site of the ribosome

B. The P site of the ribosome

C. The E site of the ribosome

D. IF1

E. IF2

BIOL308 Study Guide - Kozak Consensus Sequence, Ribosome-Binding Site, Eif2

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