BIOL483 Lecture Notes - Lecture 9: Vial, Hela, Cryopreservation
7 Sep 2013
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Assay to test invasiveness of a cell line. Is in a cell culture insert and media is bathing ontop of cell. If they are invasive they are neoplastic cells. Long term storage done with cryopreservation and uses cryovials. Cryoprotectants protectant during freezing time, otherwise you get cell damage. Prokaryotic cells lot more resistant to freezing than mammalian cells. Want it to slowly cool so u don"t get ice crystals and then warm it really quickly. Are horrible, cant see them, very small, can damage cell culture and how they grow. Smallest free living cells: many of the antibiotics target cell wall and that wont help with this. Human origin: one of the hoods is horizontal hood, perhaps u coughed and the mycoplasma could have been introduced. Number of ways u can test for it. Fluorescent dna is cheapest and easy to do. If u detect mycoplasma and that cell line is not replaceable = gotta throw it out.
