BIOL483 Lecture Notes - Lecture 4: Polystyrene, Chelation, Streptomycin
7 Sep 2013
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Important to grow cell, and allow simulation for cells to grow. Animal cells are much slower in growth when compared to bacterial or yeast cells. Penicillin in streptomycin is most common combination in addition to basal medium. Not a very strong amount about 2% of antibiotics, just to control any microbes that may grow. If your culturing technique is good, you don"t need antibiotics (not a disadvantage, but opposite of it) Shake it and it will foam up, serum will foam up. Depends on cell, but many cells that are normal tend to grow upon one layer and that"s it. Must be treated to have net negative charge on bottom so cells can be attracted to it and create attachment. Can do invidividual experiment, have cells that will grow at bottom of each well. Specialized plates treated on the bottom for cells to attach.
