Biology 1002B Lecture Notes - Cyanobacteria, Oxygen-Evolving Complex, Exonuclease
9 Sep 2013
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Paper claims that there is horizontal gene transfer from chloroplasts of vaucheria to. Manganese stabilizing protein- part of the oxygen evolving complex. Elysia has this gene that would code for this component. Amplified protien of psbo transcript: amplified= make multiple copies of it so you can see it. 5 months after feeding on algae and you are picking up transcript: suggests that gene an elysia gene not just coming from degradation of vaucheria. Diagnostic: forensic science, phylogenetic studies, disease testing. Power to amplify is very important so you can actually work with that specific piece of. Deoxynucleotides- datp, dttp, dctp, dgtp: 200um. Pcr based on specificity of primer binding. In any dna sequence: chance of finding an a, g,c,t, 1/16 of finding any dinucleotide sequence, 1/256 change for 4 base sequence, 1/ 4^16 chance of finding a given 16 base sequence. 18-24 is the range of primer too long primer might fold back on itself.
