BIOL 205 Lecture Notes - Bioremediation, Genetic Testing, Dna Profiling

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yellowchinchilla381

5 Nov 2013

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A 303 bp orf (open reading frame) has the potential to encode_______amino acids in a row: 10, 30, 100, 303, 900. This molecule represents: a protein, an amino acid always have amino and carboxyl end, a fatty acid, nucleotide. What amino acid is this: alanine, glycine, arginine, methionine, cysteine. Only two amino acids that have s (methionine: aug) and cysteine. Disulfide bonds two cysteines in close proximity will form a covalent bond disulfide bond, disulfide bridge, or dicysteine bond significantly stabilizes tertiary hair is 10-14% cysteine. Basic amino acids histones predominantly lysine and histindine histidine lysine arginine at neutral ph these amino acids are positively charges. Why is the y chromosome so small: because it has very few genes, because it has no pairing partner. Recombinations takes place between chromosomes so that if a piece of dna is lost, it can pair w/ its partner and fill the gap.

Related Questions

Type molecular weight Number in lysozyme

Alanine 89 12

Arginine 174 11

Asparagine 132 13

Aspartic acid 133 8

Cysteine 121 8

Glutamic acid 147 2

Glutamine 146 3

Glycine 75 12

Histidine 155 1

Isoleucine 131 6

Leucine 131 8

Lysine 146 6

Methionine 149 2

Phenylalanine 165 3

Proline 115 2

Serine 105 10

Threonine 119 7

Tryptophan 204 6

Tyrosine 181 3

Valine 117 6

1. The sequence of amino acids of the enzyme lysozyme is known. Below is a list of amino acids

and the number of each in the lysozyme molecule

Questions:

a. What is the average molecular weight of the 20 amino acids?

b. What is the molecular weight of lysozyme?

c. What is the average molecular weight of the amino acids in lysozome?

d. How many S-S bonds are possible in lysozyme?

(hint: look at the structures of each amino acid, and figure out which of these have sulphurs

that are FREE to form bonds).

e. Is the net charge on lysozyme positive or negative?

jadechinchilla39

SEQUANCE:

5'- GCU UGG GCU GCU GAU GCU CUU UAA CAU AGA GCU AAU AUU -3'

=======================================================================================

3- Convert your mRNA sequence into a DNA sequence, then make itdouble-stranded by writing the complementary sequence below.

4- Transcribe (write an RNA sequence) and translate (write anamino acid sequence) both the "top" and "bottom" strands startingwith the first nucleotide. You must use the genetic code in yourtextbook or in your lecture notes for this step. DO NOT use thetable above. If you encounter a stop codon, your protein sequenceis done at that point.

Use the single letter designation for amino acids as givenbelow.

Alanine (A)	Aspartic acid (D)	Asparagine (N)	Arginine (R)
Cysteine (C)	Glycine (G)	Glutamic acid (E)	Glutamine (Q)
Histidine (H)	Isoleucine (I)	Leucine (L)	Lysine (K)
Methionine (M)	Proline (P)	Phenylalanine (F)	Serine (S)
Threonine (T)	Tryptophan (W)	Tyrosine (Y)	Valine (V)

5- Use your original DNA sequence from #3. Add an A to the 5'end of the "top" strand, then write the complementary sequence onthe bottom. Add a GC to the 5' end of the

"bottom" strand, then write the complementary sequence on the top.Transcribe and translate both the "top" and "bottom" with this newsequence.

What kind of mutation(s) was introduced in the "top" strand?

What kind of mutation(s) was introduced in the "bottom"strand?

6- Use your original DNA sequence from #3. Starting at the 5'end on the "top" strand of your original sequence from part 3,delete the second A, the third G, and the fourth C along with thecomplementary nucleotides on the bottom strand. Transcribe andtranslate both the "top" and "bottom" with this new sequence.

What kind of mutation(s) was introduced in the "top" strand?

What kind of mutation(s) was introduced in the "bottom"strand?

7. Use your original DNA sequence from #3. Starting at the 5'end of the "top" strand, change the third G to an A, then changethe complementary nucleotide in the bottom. Starting at the 5' endof the "bottom" strand, change the third C to a T, then change thecomplementary nucleotide on the top. Transcribe and translate boththe "top" and "bottom" with this new sequence.

a. What kind of mutation(s) was introduced in the "top"strand?

b. Whatkind of mutation(s) was introduced in the "bottom" strand?

purplehippopotamus358

BIOL 205 Lecture Notes - Bioremediation, Genetic Testing, Dna Profiling

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