Biology 2382B Final: Cell Bio 2382 - Final Exam Notes.docx
10 Dec 2013
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*phenyl red indicator = yellow is low ph and purple is high ph (alkaline) Primary cell cultures = cells directly taken from an organism. You can plate these and grow them until they become a confluent monolayer: takes 9 days, stops growing because of contact inhibition. Treat with trypsin and transfer to new plate to continue growing: can only do 50x in humans (limitation in labs) Germ lines can grow indefinitely and do not show contact inhibition. *hela was the first germ line to be found. 3t3 mouse fibroblast (cell that synthesizes cell matrix) transformed by rous sarcoma virus. *artificially introduced a gene that turned it into a cancer cell* Asymmetric division = can differentiate into different things or. *esc are pluripotent = become any of the three germ layers but not extraembryonic things such as placenta* Not pluripotent can only differentiate into what tissue it is. Used to repair and maintain the tissue.
