CHM 2354 Study Guide - Absorption Spectroscopy, Spinach, Chlorophyll
Document Summary
Separation and analysis of chlorophyll a & b from spinach leaves. Molecular absorption spectroscopy1 can be employed in the lab to deduce concentration of a compound in a sample based on its absorption in comparison to a reference standard. Here we attempt to undergo an analytical analysis of the chlorophyll a/b content of a representative sample of spinach leaves. The analysis was only performed on the spinach leaves themselves, and excluded veins and stems. Chlorophylls a & b3 were extracted using aqueous acetone and their absorbance measured at two wavelengths, 645nm & 663nm. The uniformity of the sampling technique was to be confirmed using anova statistical methods. 67. 5% of the spinach samples on average, and chlorophyll b constituted 32. 5% of the spinach samples on average. For method and procedure please refer chm4116: advanced instrumental analysis laboratory manual. Samples were prepared using analytical balances for weight. Table. 2: chlorophyll a & b concentrations in spinach leaves.
