BIOL 2021 Study Guide - Midterm Guide: Fluorescent Tag, Dapi, Phosphatidylcholine
17 Sep 2014
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Biol2021 test 1: version a: you are completing version a. Bubble in a beside #1 on your scantron. While observing it under the fluorescence microscope, you carry out a frap (fluorescence recovery after photobleaching) experiment by bleaching a patch of your labeled proteins with a high-powered laser and then watching what happens. Biol2021 test 1: version a: identify the molecule below, phosphatidylethanolamine, phosphatidylserine, phosphatidylcholine, sphingomyelin, cholesterol, glycolipid, the lab you are working in conducts research on a protein that is known to localize on the lateral plasma membrane. Which letter corresponds to the membrane being hyperpolarized: a, b, c, d, e. Biol2021 test 1: version a: the lipid composition of the plasma membrane is asymmetric. Tom: tom cleaves off the signal sequence after the protein is translocated, a and b only, a genetically altered protein has two conflicting signal sequences. A nuclear localization/import signal and an er start-transfer signal.
