MGY377H1 Midterm: Study notes for midterm
Document Summary
C, h, o, n, s, p, k, etc: elements found in form of water, organic and inorganic ions, small molecules, macromolecules that have structural and functional roles in cell. Chemoorganotrophs use organic form of carbon. Other macronutrients include nitrogen, p, s, k, mg, ca, na, - with essential functions in cell. Required in small amounts by cells because they fulfil specific roles in biosynthesis. Three categories: purines and pyrimidines, amino acids, vitamins. Allows identification, characterization, establish links to disease. Medium must contain all required nutrients, correct growth environment (temperature, oxygen concentration) Most bacteria on earth cannot be cultured. Liquid media to grow pure batch cultures. Solidified media to isolate pure cultures, to estimate viable bacterial populations, and other purposes: use agar. Chemically defined media exact chemical composition is known. Complex media exact chemical composition unknown, usually containing digests of animal or plant products: considered rich medium.