CSB351Y1 Lecture 18: Lecture 18

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redskunk879

15 Feb 2012

School

UTSG

Department

Cell and Systems Biology

Course

CSB351Y1

Professor

Mounir Abou Haidar

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Document Summary

*details of prion movie shown from 1-2pm on this day are omitted. I will go over the polioviruses first, then i will go back to rhinoviruses and hepatitis a virus they are all picornaviruses. Polio viruses are part of the picornaviridae (mammalian viruses) which includes. Enteroviruses [polio, coxsackie virus, and enteric cytopathogenic human orphan (echo)]. Cardioviruses [encephalymyocarditis virus (emc) it will hit the brain and the heart] Aphtoviruses [foot and mouth disease mostly an animal virus found in the. Uk~ know a bit about it but this is an animal virus. It is not pathogenic for humans it will only cause a mild rash on the hands and arms. They are ss (+)rna and they have a cellular receptor on chromosome 19 the icam-1 (icam=intracellular adhesion molecule). They have a poly-a tail, are cap independent and translation occurs via ires (ires=internal ribosome entry site). Picornaviruses do not produce subgenomics as they have one orf.

Related Questions

You are a Picornavirus researcher who is trying to make a name for yourself. You have developed a drug that strongly inhibits certain Picornavirus infections. After getting very excited about it, you are dismayed to find a strain that is resistant to the drug. You arenât completely sure how the drug actually works, but it deals with lipid biogenesis. You suspect that itâs involved with formation of the viral factories (the Picornavirus field calls these âROâs or replication organelles).

The virus is an Encephalomyocarditis virus (EMCV). You name the mutant EMCV-mutA. For the first step, you wanted to see if the ROs formed at all. You infected cells with EMCV, EMCV-mutA, and mock infect. Both viruses produce the expected punctae (distinct dots) while viewed using immunofluorescence.

1) Please address each viral protein and why it may or may not be the problem. That is to say, if the expected punctae are being formed for both the WT and the mutant, and if you think this involves the ROs, where might the mutation be that allows this new virus to evade the drug?

2Apro

3Dpol