BIOL 2020 Chapter Notes - Chapter 4: Affinity Chromatography, Enzyme Assay, Differential Centrifugation
Document Summary
Proteins must be separated from all other constituents of cell so their biochemical properties can be identified and characterized ; aka protein must be purified. 40. 1 the proteome is the functional representation of the genome. Completely sequenced human genome contains 3 billion bases and about 25000 genes. Proteome level of functional information, which encompasses types, functions and interactions of proteins that yield a functional unit. Genome provides list of gene products that could be present but only a subset of these gene products will actually be expressed in a given biological context. Proteome tells us what is functionally present ; not a fixed characteristic of cell. Proteome varies with cell type, developmental stage and environmental conditions. 40. 2 the purification of proteins is the first step in understanding their function. To understand a protein, we need to isolate it from the thousands of other proteins in the cell.
