BPS 3101 Study Guide - Final Guide: Gel Electrophoresis, Southern Blot, Bamhi

• School: University of Ottawa
• Department: Biopharmaceutical sciences
• Course: BPS 3101
• Professor: Linda Bonen

Document Summary

Dna endonucleases cut double stranded dna at specific recognition sites (often palindromic ( same when read 5"to3" or 3"to5") Recognition sequences often 4 or 6bp, but also rare cutters can be useful for generating very large fragments in genomic mapping. They produced blunt and sticky ends (bamhi produces sticky ends) Exonnucleases remover nt at end of dna/ rna. Two different restriction enzymes may generate same sticky ends: bamhi and sau3ai cute 5"-3" gatc and produc sticky ends. Isoschizomers- restriction enzymes with identical recognitions sequences. Example using isoschizomers to assess dna methylation state of genes in cancer patients. Agarose gel electrophoresis- to separate dna fragment by size. The smaller the fragment the faster is travels, therefore it travels furthest. Dna is negatively charges and therefore loaded on the negative side and travels to the positive end. It is useful when separating out relatively smaller fragments: pulse fiels electrophoresis is used for separation of large fragments/ whole chromosomes/megaplasmids.