shakti8630

shakti8630

Lv7

Uttarakhand Technical University

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my name is shakti rana and i am from up and i am bachler student.

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Answer: Step-by-step explanation: A) The domain of the function is the set of ...

Design an experiment in which you will test theeffect of an acidic fluid on enzymatic activity. (Recall: enzymesare proteins.) To complete this project, it may be useful for youto review theScientific Method Tutorial (found theCourse Content section of the classroom under the Science LearningCenter link) and the Scientific Method lab (Lab 1), so thatyou can better understand how to design an experiment. It may alsobe helpful for you to review Lab 4 (Enzymes). As you review Lab 4,you will be reminded that there are several factors that impactenzymatic activity: pH, temperature, and amount of reagent. Feelfree to refer to observations and information from Lab 4 as youcomplete the questions required in the Final Applied Project (seethe questions below).

As you design your experiment for this project,please remember that you are trying to examine how an acidic fluidwill modify the outcome of an enzymatic reaction. To successfullycomplete this project, you will need to identify the question(s)being asked in your experiment and the hypothesis that you aretesting. In your experimental design, you must clearlyexplain what you are doing. That means that you will need toidentify the enzyme and the acid, as well as explain yourexperimental protocol (this information will help you to answerquestion 2). You must also thoroughly explain how the addition ofthe acidic fluid impacted the overall reaction process (thisinformation will help you to answer question 4).

Hint: Keep in mind that the acid will changethe environmental conditions of the experiment (for example, a lowpH value could change the shape of the active site on the enzymeprotein), without directly participating in thereaction.

Lab Materials

You may need the following, depending on yourexperimental design.

Materials in your lab kit:

  • none

Additional materials you may need:

  • plastic beakers or cups
  • hydrogen peroxide solution (from Lab4)
  • yeast (from Lab 4)
  • sample of fresh meat, about 1 cm cubed insize (unprocessed and uncooked, e.g., liver, steak, fish, orpoultry%u2014particularly organs)
  • sample of fresh vegetable, about 1 cmcubed in size (e.g., potatoes or other root vegetables)
  • a pen for labeling the beakers orcups
  • a ruler with centimeter markings

Remember, the goal of this project is for you toevaluate the effect of an acid on enzymatic activity. As part ofthis assignment, you must identify your source of enzyme and theacid used. As you work though this project, you must also make surethat the following questions/points are addressed:

1. What question are you asking? Clearly state yourhypothesis.

2. Design an experiment. Provide a detailed account of thematerials and methods used to conduct the experiment. Also includethe methods for data collection and analysis.

3. Conduct the experiment and record your results below. Whatdid you observe? Which samples showed bubbling?

4. Use your knowledge about factors that effect enzymaticactivity to interpret your results. It may be necessary for you torefer to your lab manual and/or other resources. What effect didthe acid have on the enzyme?

5. Was your hypothesis supported? What is your conclusion?Explain your answer.

6. Cite all references used to complete your Final AppliedProject.


Answer: Step-by-step explanation: What question are you asking? Clearly state ...

Design an experiment in which you will test theeffect of an acidic fluid on enzymatic activity. (Recall: enzymesare proteins.) To complete this project, it may be useful for youto review the Scientific Method Tutorial (found the Course Contentsection of the classroom under the Science Learning Center link)and the Scientific Method lab (Lab 1), so that you can betterunderstand how to design an experiment. It may also be helpful foryou to review your textbook and Lab 4 (Enzymes). As you review Lab4, you will be reminded that there are several factors that impactenzymatic activity: pH, temperature, and amount of reagent. Feelfree to refer to observations and information from Lab 4 as youcomplete the questions required in the Final Applied Project (seethe questions below).

As you design yourexperiment for this project, please remember that you are trying toexamine how an acidic fluid will modify the outcome of an enzymaticreaction. To successfully complete this project, you will need toidentify the question(s) being asked in your experiment and thehypothesis that you are testing. In your experimental design, youmust clearly explain what you are doing. That means that you willneed to identify the enzyme and the acid, as well as explain yourexperimental protocol (this information will help you to answerquestion 2). You must also thoroughly explain how the addition ofthe acidic fluid impacted the overall reaction process (thisinformation will help you to answer question 4).

Hint: Keep in mind thatthe acid will change the environmental conditions of the experiment(for example, a low pH value could change the shape of the activesite on the enzyme protein), without directly participating in thereaction.

LabMaterials

You may need thefollowing, depending on your experimental design:

Materials in your labkit:

Answer: Step-by-step explanation: Experiment Design: Question: How does the ad...

Design an experimentin which you will test the effect of an acidic fluid on enzymaticactivity. (Recall: enzymes are proteins.) To complete this project,it may be useful for you to review the Scientific Method Tutorial (found the CourseContent section of the classroom under the Science Learning Centerlink) and the Scientific Method lab (Lab 1), so that you canbetter understand how to design an experiment. It may also behelpful for you to review Lab 4 (Enzymes). As you review Lab 4, youwill be reminded that there are several factors that impactenzymatic activity: pH, temperature, and amount of reagent. Feelfree to refer to observations and information from Lab 4 as youcomplete the questions required in the Final Applied Project (seethe questions below).

As you design yourexperiment for this project, please remember that you are trying toexamine how an acidic fluid will modify the outcome of an enzymaticreaction. To successfully complete this project, you will need toidentify the question(s) being asked in your experiment and thehypothesis that you are testing. In your experimental design, youmust clearly explain what you are doing. That means that you willneed to identify the enzyme and the acid, as well as explain yourexperimental protocol (this information will help you to answerquestion 2). You must alsothoroughly explain how the addition of the acidic fluid impactedthe overall reaction process (this information will help you toanswer question 4).

Hint: Keepin mind that the acid will change the environmental conditions ofthe experiment (for example, a low pH value could change the shapeof the active site on the enzyme protein), without directlyparticipating in the reaction.

please address the following:

1. What question are you asking?Clearly state your hypothesis.

2. Design an experiment. Provide adetailed account of the materials and methods used to conduct theexperiment. Also include the methods for data collection andanalysis.

3. Conduct the experiment and recordyour results below. What did you observe? Which samples showedbubbling?

4. Use your knowledge about factorsthat effect enzymatic activity to interpret your results. It may benecessary for you to refer to your lab manual and/or otherresources. What effect did the acid have on the enzyme?

5. Was your hypothesis supported? Whatis your conclusion? Explain your answer.

6. Cite all references used tocomplete your Final Applied Project.


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