Enzymes are sensitive to pH and temperature. What would happen to the lactase enzyme if it was heated at a high temperature? Predict the outcome of the reaction with lactase and Tactose.

The enzyme lactase converts the disaccharide lactose into the monosaccharides glucose and galactose.

To test its activity a researcher puts 1ml of 1mg/ml lactase into a 5ml volume of 1mg/ml lactose and measures the amount of glucose at the end of a five minute time period. During the experiment the temperature is set to 37C. He repeats the experiment at pH 6, 7 and 8 and then creates a graph of the data.

In the following experiment indicate whether each of the following is a dependent, independent (experimental), or controlled variable (if it is none of these select that instead).

glucose concentration

[ Choose ] independent controlled dependent none of these

lactase concentration

[ Choose ] independent controlled dependent none of these

oxygen in ml

[ Choose ] independent controlled dependent none of these

temperature

[ Choose ] independent controlled dependent none of these

pH

Recently we have started suspecting that one of our children hashypolactasia (lactose intolerance), and so accordingly I have hadmy first exposure to lactose-free dairy- and dairy-like products.In addition to soy milk and almond milk, we have recently purchasedactual dairy milk that was labeled lactose-free. This gave me theimpression that they had used some process to remove the lactose,but looking at the ingredients more closely, it seems that theysimply added lactase enzyme to the milk to assist breaking down thesugars.

My question is this: when is the lactase enzyme active? Does thelactase activity begin right as it is added to the milk, or will itnot become active until ingested?

Essentially, I'm wondering if this type of milk can/should beused in cooking or baking. High temperatures will certainlydenature the lactase enzymes in the milk, but if the lactose hasalready been broken down, this shouldn't be a problem.

QUESTION 3

What is the purpose of chromatin-remodeling enzymes in human cells?