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miuLv1

24 Nov 2021

Explain one other experimental method for identifying the isoelectric point of a protein.

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Explain why precipitation takes place at the isoelectric point of an amino acid or protein.

A. Design an experiment to purify protein X on an anion-exchangecolumn, Protein X has an isoelectric point of 7.0.

B. How would you purify protein X wing ion-change chromatography ifit turns out the protein is only stable at a pH between 6 and 6.5?

scarletraccoon142

Short answer questions:

1. Explain why precipitation takes place at the isoelectric point of an amino acid or protein.

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Explain one other experimental method for identifying the isoelectric point of a protein.

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