BIOL 467 Study Guide - Final Guide: Keratin, Doxycycline, Motility
Document Summary
Wanted to prove emt model: stationary epithelial tumour cells convert into motile mesenchymal cells to metastasize, show that his model is reversible (by twist 1 activation/deactivation) Why was this question important to the field. Twist1 expresed at primary tumour sites but not the secondary distant metastases (more twist1 expression at primary site then at lymph nodes (secondary site)) [6] Cancer cells (identified by keratin marker) invaded into the basilar membrane, suggesting that twist1 is resulting in the degradation of the ecm, which would allow these cells to migrate and enter the blood stream before establishing secondary tumours [9] Mice receiving oral dox developed secondary tumours. Both oral and topical doxycycline treatment resulted in much lower expression of e-cad in primary tumour sites, compared to secondary. This means that they are turning off their epithelial characteritics at the primary and in the secondary they are reverting back to their epithelial characteristics [12]
