BIOL 200 Study Guide - Quiz Guide: Guanine, Dna Replication, Ribozyme

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Published on 26 Nov 2012
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1
.
The tRNA used to prime the reverse transcriptase reaction during the conversion of the
retroviral RNA genome to a double-stranded DNA genome
Student Response
Value
Correct Answer
A.
is encoded by the retrovirus
B.
is also used during the transcription of the retroviral reverse transcriptase gene
C.
is normally used during the translation of host-encoded protein-coding transcripts
100%
D.
synthesized using dNTPs
E.
two of the above
Score:
1/1
2
.
In a standard PCR protocol, which is the correct order of steps in each cycle? a. annealing of
the primers b. denaturation of the double-stranded DNA template c. extension by taq DNA
polymerase d. removal of the primer by FEN-1
Student Response
Value
Correct Answer
A.
a, c , b, d
B.
c, b, a, d
C.
b, a, c
100%
D.
c, a, b, d
E.
a, b, c
1/1
3
.
Use the figures below to answer the following question. In order to clone gene “X (black
rectangle) from the SV40 genome that was digested (i.e. "cut") to completion with the
restriction endonucleases EcoR1, Sph1 and Pst1, the plasmid vector must also be digested
with
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Student Response
Value
Correct Answer
1.
Pst1 and Sph1
2.
EcoR1 and Pst1
100%
3.
EcoR1 and Sph1
4.
EcoR1 only
5.
EcoR1, Pst1 and Sph1
Score:
1/1
4
.
Which of the following does not involve gap repair?
Student Response
Value
Correct Answer
1.
insertion (i.e. "paste") of a DNA transposon
2.
base excision repair
3.
nucleotide excision repair
4.
proofreading by DNA polymerase delta
100%
5.
two of the above
Score:
1/1
5
.
Which of the following DNA repair mechanisms relies on differentiating the parental and
daughter strands immediately after DNA replication?
Student Response
Value
Correct Answer
1.
error-prone double-stranded break repair by end joining
2.
base excision repair
3.
mismatch excision repair
100%
4.
nucleotide excision repair
5.
two of the above
Score:
1/1
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6
.
Reverse transcriptase has which common feature(s) with DNA polymerase alpha?
Student Response
Value
Correct Answer
A.
can only use DNA as a template
B.
does not require a primer
C.
incorporates dNTPs
100%
D.
synthesis occurs 3’ to 5
E.
all of the above
Score:
1/1
7
.
Deamination of methyl-cytosine leads to the formation of
Student Response
Value
Correct Answer
A.
cytosine
B.
guanine
C.
uracil
D.
adenine
E.
thymine
100%
Score:
1/1
8
.
How many types of fluorescently-labeled reaction products will be produced in the following
DNA sequencing reaction?
Reaction tube contains DNA polymerase, dNTPs (100 mM), fluorescently-labeled ddGTP (1
mM), primer (see below) and template (see below). DNA polymerase, primer and template
are not rate limiting in this reaction (i.e., are present in excess in the reaction).
primer = 5’-CTTGGCTACTGCATTGGTC-3’
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