CHY 183 Study Guide - Final Guide: Neutron Activation Analysis, Thermal Cycler, Amido Black 10B
16 Jul 2017
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Movies after midterm: planted evidence dna ch 15. Advantage no prior knowledge of individual species or type of organism is needed, just extract dna. Method used to obtain and compare genetic fingerprints or profile of species or strains. Beans need to be extracted from tough outer shell or pod, put into liquid nitrogen. Dna segment is too short so pcr technique is used to copy the dna using dna thermal cycler, ih hours have millions of copies. Dna placed in various lanes of a gel, colour dye is added and the dna is subject to an electrical field. Electrical current draws negatively charged dna fragments to positive end. Results resemble bar code, genetic fingerprint of palo verder. Applied pcr to it; pcr can be applied with rapd. First time plant dna used to convict person, first time plant dna admissible in court using frye standard. No two plants are alike- dna in plants is unique.
