MBB 222 Study Guide - Final Guide: Dna Polymerase Iii Holoenzyme, Recombinant Dna, Dna Gyrase

• School: Simon Fraser University
• Department: Molec Biol & Biochem
• Course: MBB 222
• Professor: Edgar Young

Document Summary

Mbb222 final exam review: dna replication and recombinant dna methods. Dnaa = recognizes the ori sequence and opens the duplex at specific sites. Dnac = assists dnab binding at the origin. Dam methylase = methylates gatc sequences at the oric. Dna pol iii = dna strand elongation. Dna pol i = fills gaps in sequences and excises rna primers. Polylinker (multiple cloning sites) for inserting cloned dna. System for regulating transcription of the inserted dna (via bacterial promoter, operator and genes encoding repressor) Transcription termination sequence at the end of the insert. Ribosome-binding sequence at the start of the insert. Can contain sequences to allow production of fusion or tagged proteins. Typically, the cdna version of the foreign gene is used in an expression vector. Recombinant plasmids are selected for via selection markers that select for positively transformed cells. Positive selection: the cell survives and growth is permitted. Consider the transformation of the gene for beta-lactamase.