MBB 331 Final: MBB 331 3rd exam notes

• School: Simon Fraser University
• Department: Molec Biol & Biochem
• Course: MBB 331
• Professor: onlinecourse

Document Summary

Interactions with basal apparatus: eukaryotic transcription factors do not directly contact polymerase (unlike prokaryotes, activation domains of eukaryotic transcription factors interact with basal apparatus thru contact with general (tfiix) factors often tfiib, tfiia, or tfiid, activation domains promote formation of basal apparatus. Repressors block basal apparatus assembly: transcription activators > assemble basal transcription apparatus, transcription repressor > blocks basal apparatus assembly eg. dr1 repressor prevents tfiib from joining tfiid complex > assembly blocked > transcription initiation blocked, eukaryotic repressors block assembly whereas bacterial or phage repressors interfere with rnap activity. Common response elements:2 examples of these (1) hse: heat shock response element in promoters & bound by hstf ***be able to recall this: will drive the transcription of genes that are required to protect the cell against heat shock heat will cause protein to denature. (lead and alcohol is another toxic thing for your body) (2) gre: glucocorticoid response element enhancer recognized by steroid receptor, binds steroid to activate transcription.