BIOL107 Study Guide - Final Guide: Vacuole, Numerical Aperture, Oil Immersion

• School: University of Alberta
• Department: Biology (Biological Sciences)
• Course: BIOL107
• Professor: Frank Nargang

Document Summary

Pure culture an isolated group of genetically identical cells. Colony a small mass of genetically identical cells. Medium a solution or solid that contains nutrients that cells can use to grow. Magnify and project the image to the body tube. Focuses light from the source onto the specimen. Improves contrast by enabling the user to decrease the amount of light on the specimen. Contains a prism that refracts the light from objective to ocular lens. Specimen size: (ocular divisions covered by specimen) x (ocular micrometer calibration) (fov diameter) x (proportion of field of view) The capacity to distinguish two points under a microscope as distinct. Resolution value is defined as the minimum distance required between two points in order to view them as distinct. Where n. a. is the numerical aperture of the objective lens. Using immersion oil (reduces scattering and increases numerical aperture) The difference in intensity between an object and its surrounding medium.