GENET390 Study Guide - Midterm Guide: Restriction Enzyme, Phosphodiester Bond, Endonuclease

• School: University of Alberta
• Department: Genetics (Biological Sciences)
• Course: GENET390
• Professor: George Owttrim

Document Summary

Renaturation: nucleation + zippering, add salt and heat for speed and accuracy, modification marking dna to not be cut. Genet 390 personal condensed notes up to midterm i: needs mg2+ and sam, type iii res, cleave @ collision of 2 identical, non-palindromic recognition sites, chloramphenicol can be added to stringent plasmids to cause. Uncontrollable replication: phage ori: you can make ss copies of plasmid in this way if you add phage dna. Genet 390 personal condensed notes up to midterm i: melting temperature, why is thymine used in dna instead of uracil, because cytosine can be deaminated into uracil. Normally, this mistake would be fixed in dna because uracil doesn"t exist in dna. L-shape structure formed in solution from watson-crick trna clover leaf structure: rna evolved before dna. Genet 390 personal condensed notes up to midterm i: rna can be involved in regulation, non-coding rna, effect of ph on dna and rna, acid acid hydrolysis of glycosidic bond (acid depurination)