MICRB265 Study Guide - Final Guide: Multilocus Sequence Typing, Open Reading Frame, Great Oxygenation Event

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Koch"s postulates: principles which proved cause of disease. Disease-causing organism present in animals suffering but not in healthy animals. Organism must be cultivated in a pure culture away from animal body. Isolated organism must cause disease when inoculated in healthy susceptible animals. Organism must be isolated and cultured again, then compared with original organism. Must grow in pure culture (if it requires complex nutrients, will fail) If it doesn"t grow on selective = will fail. Pure culture isolation developed by fannie hesse and richard petri. Waksman and schatz streptomycin in soil bacteria. Brock bacteria in hot springs, isolated source of taq polymerase (thermus aquaticus) Linnaeus invented naming system (genus, species: ribosomal rna quantitative measure of evolution and relatedness of all life forms, 16s rrna in prokaryotes, mitochondrion, chloroplast, 18s rrna in eukaryotes, pcr, and analysis used to determine evolutionary relationships. Prokaryotes are much smaller in size (mitochondrion size)

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