ZOOL344 Study Guide - Final Guide: Statistical Hypothesis Testing, Immunoglobulin G, Immunocytochemistry

• School: University of Alberta
• Department: Zoology (Biological Sciences)
• Course: ZOOL344
• Professor: John Chang

Document Summary

Lab #1: subcellular fractionation of mitochondria: enzyme: succinic dehydrogenase (sdh, located on the inner membrane of the mitochondria. Involved in etc: removes hydrogen from succinate and transfers to the tetrazolium compound, activity of enzyme can be observed using colour agents, tetrazolium salts. Reduction products: water-insoluble formazans: substrate: tetranitro blue tetrazolium. Reddish-purple formazan is immobile: using rat liver, rinse with sucrose solution, phosphate buffer. Discard pellets both times: 1 ml of tnbt and sodium succinate, mitochondrial vs. microsomal fraction. Frequency distribution should be shifted in favour of stages 1 and 2 at higher concentrations: molecular activity ratio (m. a. r. ) calculation, phentolamine = antagonist, phenylephrine = agonist. Lab #5: trout (goldfish really) digestive tract: part a: gut bag experiment. Weight change = water fluxed from mucosal to serosal surface (or vice versa. Glucose + atp ------ (hexokinase) --------> glucose-6p + adp. Not: procedure and materials, used cdna template generated from trna, tested stomach, pyloric caeca, midgut, hindgut, etbr for agarose gel.