MICR 3230 Final: TCRs and signaling notes

• School: University of Guelph
• Department: Microbiology
• Course: MICR 3230
• Professor: Azad Kaushik

Document Summary

Chapter 3 - t cell receptors and signaling. Harder to determine tcr biochemistry (no soluble form) Made hybridomas (fusion product of 2 cells) Immunized mouse w. ova (creaing t cells speciic for ova) then made b cells (anibodies) that sees these t cells as anigen! The anibodies are in compeiion with the ova anigen for the tcr, because when they are present, the t cells don"t proliferate. Proved the tcr is a single receptor for mhc and ag. Heterodimer: most possess an chain and a chain. Recognize short pepide in binding groove of mhc on apc surface. : bind pepides presented by mhc i or ii more innate, higher expression in ruminants and pigs. : found in mucosal issues and recognize diferent anigens than , Variable and constant domains held together via disulide bonds. Variable domains (amino terminus) bind the epitope, and are deined by v , v ,