BIOL308 Study Guide - Final Guide: Semiconservative Replication, Hydrogen Bond, Phosphodiester Bond
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Chapter 10
1.Outline the history of our knowledge on DNA up to Watson and Crick. What were the main contributions made by each researcherâs key experiment?
2.Explain the setup of the Hershey and Chase experiment, what would the results have been if protein was the genetic material?
3.Draw the structure of a DNA nucleotide, labeling each main component correctly. How does an RNA nucleotide differ?
4.If a section of double stranded DNA contains 19% Adenine, how much Thymine is present?
5.You are a researcher studying the genetic basis of heart attacks and have been working to determine the expression levels of different genes that might contribute to cancer formation. You obtain the DNA methylation status of five genes of interest (the data are shown in the table below). The plus (+) sign indicates the level of DNA methylation; more plus signs correlates with increased methylation levels.Based on this information which genes would you predict to have the highest rate of transcription?
| Gene | Methylation levels |
| 1 | ++ |
| 2 | +++++ |
| 3 | +++ |
| 4 | ++ |
| 5 | + |
What are the characteristics of the 3 main DNA forms?
Chapter 11
What are the different types of chromatin?
What are the structures and important roles for telomeres and centromeres?
What are the differences found between eukaryotic chromosomes and mitochondrial?
Chapter 12
Explain each of the different models of replication.
If you grow a culture of bacteria in media with radioactive nucleotides so that all DNA in the cells include radioactive nucleotides and then place the bacteria in new non radioactive media. After two rounds of replication what proportion of the DNA molecules will contain radioactivity?
Summarize the similarities and differences between rolling-circle replication, theta replication and linear eukaryotic replication.
What are the functions of the different DNA polymerases found in eukaryotic cells?
Draw a replication fork and include all key components and orientations. (Leading/lagging strands, DNA helicase, RNA primer and DNA gyrase)
What is the Holliday model of recombination and what are the necessary steps?
Chapter 13
What are the different types of RNA and what roles do they play?
Describe the properties and functions of each of the RNA polymerases and how they differ depending on the organism.
Describe in detail the process and mechanisms of transcription in both prokaryotes and eukaryotes.
Chapter 14
What are the primary purposes of each of the three post transcriptional modifications that occur in eukaryotic cells.
What is alternative splicing and what role does it play in the cell?
How is ribosomal RNA processed after transcription?
How do siRNA and miRNA work, describe/draw out the process in detail.
13. The arms of a human and the wing of a bat are _________________ structures, composed of the same bones that have been modified for different functions.
| a. | analogous |
| b. | vestigial |
| c. | homologous |
| d. | convergent |
14. Xeroderma pigmentosum is an autosomal recessive genetic disorder. People who are homozygous for the disease-causing allele have skin that is damaged by exposure to ultraviolet radiation; this can lead to skin cancer at an early age. The mutation that causes this disorder is in a gene that functions:
| a. | to repair DNA by excising (removing) nucleotides damaged by UV light. |
| b. | in lysosomes, to break down lipids that otherwise accumulate in the nervous system. |
| c. | to produce the UV blocking pigment melanin in the skin cells |
| d. | in red blood cells to carry oxygen to the skin. |
15. The Amish are a religious sect descended from a small group of colonists who came to the United States over 200 years ago. Their religious beliefs have kept them isolated from society. The Amish have a much higher than average incidence of both polydactyly (extra fingers and toes) and dwarfism in their communities. This is most likely explained by:
| a. | inheritance of acquired characters. |
| b. | the founder effect. |
| c. | disruptive selection. |
| d. | natural selection. |
16. In the structure of DNA that Watson and Crick proposed, the sides of the "ladder" of the DNA molecule are always the same distance apart, making the molecule a constant width. This occurs because when the bases hydrogen bond to form the rungs of the "ladder", a:
| a. | purine always pairs with a pyrimidine. |
| b. | purine always pairs with a purine. |
| c. | pyrimidine always pairs with a pyrmidine. |
| d. | The structure of the double helix is not determined by how the bases pair. |
17. The RNA transcript of DNA that travels to the cytoplasm, carrying the instructions to make a protein, is called:
| a. | rRNA. |
| b. | tRNA. |
| c. | mRNA. |
| d. | RNA polymerase. |
18. When DNA is replicated, the error rate is approximately one error for every 10,000 nucleotides copied. However, that error rate is reduced to only 1 error for every 1 billion nucleotides. The DNA sequence is "corrected" by:
| a. | repeating S phase to see if the error rate is lower the second time around. |
| b. | enzymes that proofread the DNA and repair errors. |
| c. | messenger RNA during the process of transcription. |
| d. | Any errors made during DNA replication cannot be corrected. If errors are detected after S phase, the cell is destroyed. |
19. Mendel's principle of segregation says that:
| a. | when gametes are formed, each gamete receives only one allele for a particular gene. |
| b. | some genes are dominant to others. |
| c. | a testcross must be used to determine the genotype of an organism with a dominant phenotype. |
| d. | all of the above |
20. Tay-Sachs disease is lethal before reproductive age, but the allele persists in Louisiana French Canadians because:
| a. | it is a dominant allele. |
| b. | new mutations causing this disease are common in that population. |
| c. | it is not expressed in the phenotype of heterozygous individuals. |
| d. | the disease is contagious and can be transmitted by nonheritable means. |
21. Physical features that are similar in organisms which are not closely related to each other are most likely a result of ____________. A good example of this is the similarities seen between some marsupial and placental mammals.
| a. | natural selection. |
| b. | adaptation to similar environments |
| c. | convergent evolution |
| d. | All of the above phrases can be used to correctly fill this blank. |
22. In humans, which of the following sex chromosome compliments could be found in males?
| a. | XY |
| b. | XX |
| c. | Y |
23. The proteins that are associated with eukaryotic chromosomes:
| a. | help to package the DNA within the nucleus. |
| b. | are involved in gene expression, through binding with the DNA. |
| c. | may be replicating or transcribing the DNA. |
| d. | All of these are true of proteins that are associated with eukaryotic chromosomes. |
24. A sequence of DNA nucleotides that contains the information to produce a single protein is a(n):
| a. | codon. |
| b. | gene. |
| c. | polypeptide. |
| d. | anticodon. |
