BIOL432 Study Guide - Final Guide: Lipase, Methyltransferase, Antibiotics
14 Apr 2015
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Chapter 13: synthesis of commercial products by recombinant microorganisms the production of low molecular weight compounds such as: vitamins, amino acids, dyes, precursors of biopolymers, antibiotics. 13. 1 gram ve organisms have an added layer of protection from ren other than methylation but localizing their ren within the periplasmic space and the modification enzyme in the cytoplasm: provides a cellular defense against attack by foreign dna (virus) if cloned together (close to each other on the chromosome) the modification enzyme must be expressed prior to the ren. Resistance to lytic action of dna bacteriophages was selected for: resistant transformants were grown and osmotically shocked to release the periplasmic proteins which could be assayed for psti ren activity, positive clones were then assayed for psti methylase activity, one clone contained an intact psti endonuclease and methylation operon including the p. stuartii promoter (cid:224) the methylation enzyme preceded the endonuclease.
