BIOL483 Study Guide - Final Guide: Streptokinase, Cytoskeleton, Sertoli Cell
7 Sep 2013
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Hemocytometer: manual way of counting cells, neubauer is the most commonly used. Electronic counter or particle counter: electronic way, coulter counter. Known volume of cell suspension buffered into saline solution. Solution drawn up through a suction hole. As particles travel into hole it passes 2 electrodes, one inside, one outside. This creates pulse that is detected by counter, can set upper and lower size limits of particles and eliminate particles that are not the size of the cells you are counting. Undergo fixation: must be fixed because that way you get less clumping of the cells, fixation is where a cell is no longer capable to divide. Protein determination: measures total amount of protein in cell culture, uses bradford assay. Lyse cells, add naoh + comassie blue which causes blue colonies to form. Compare it to standard results on specto.
