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205 Lab 1 Review.docx

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Department
Kinesiology
Course
KIN 205
Professor
James Rush
Semester
Fall

Description
205 Lab 1 Review StevenIntroSkeletal muscle is malleable remodeled in response to environment stimulus3 schemas used to classifyoContractile Properties Determined by myosin ATPase activity MHCoContractile and Metabolic CharacteristicsoContractile and Fatigue CharacteristicsMuscle BiopsyoSample obtained from superficial muscle and analyzedoIf used for metabolites frozen in liquid nitrogenoSample contains 300600 fibresoCross sectioned 1016 um thick for analysis perpendicular to alignment of fibresHistochemical StainingoUse of dyes to stain for properties of cellular componentsoEnzymatic reactionsoStain specific products of chemical reactions and form precipitateFibre TypingoMeasure myosin ATPase activityoHeavy chain expression determines myosin ATPase activityoHeavy chain mATPases are pH sensitive allows determinationMammalian Skeletal Muscle Fibre TypesType IType IIAType IIXType IIBMATPaseNot in HumanspH103 basicLightDarkDarkMedium43 acidicDarkLightLightLight46DarkLightMediumDarkMetabolic StainsNADH test of DarkMediumLightMedLightoxidative capacityLightLightMedMediumDarkGPDH test of glycolytic capacity
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