MICB 201 Study Guide - Midterm Guide: Enol, Depolymerization, Ciprofloxacin

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greybeetle348

22 Jun 2016

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They disrupt a cellular process or structure in the target organism without disrupting the same process of structure in the producing organism. Most antibiotics interfere with: peptidoglycan synthesis, translation, transcription or dna replication. A few directly disrupt membranes: using the process of peptidoglycan synthesis explain how -lactam antibiotics and vancomycin work to kill bacteria. To add new peptidoglycan, bonds have to be broken in the pre-existing mesh, autolysins do this. They catalyze the breaking of the covalent o-glycosidic bonds between the nam and nag sugars creating a free end to add new pg. Transglycosylase enzyme (tg) catalyzes the joining of nam and nag extending the glycan chain. Transpeptidase enzyme (tp) catalyzes the x-linking of the glycan chains via their tetrapeptides. Penicillin prevents x-link formation by binding to and inhibiting the transpeptidase enzyme. Therefore, the new pg does not have x-links.

Related Questions

During the golden age of antibiotic discovery, many new antibiotics were isolated from the soil. Recent studies have found that soil bacteria are naturally resistant to most antibotics. Some of the soil bacteria may even use antibiotics as a nutrient source. Antibiotics must kill a broad spectrum of bacteria while being absorbed harmlessly by the body. Most antibiotics were developed based on Ehrlich "magic bullet" paradigm which is suited to testing antibiotic efficacy in a lab. However, infections are more complex than a pure culture on media in a petri plate. Antibiotic resistance conjures up the pre-antibiotic era of medicine in the early 20th century. The emergence of a plasmid mediated resistance to carbopenems, a last-line drug against a variety of bacteria was alarming to public health workers. Antibiotics may cause collateral damage like a C. diff infection that leads to colitis.

5. Why is a bacterial infection that involves a biofilm harder to control with antibiotics than an infection which does not involve a biofilm?

6. Explain how taking antibiotics would cause the bacteria Clostridium difficle, which is part of the healthy colon microbiome, to become pathogenic?

7. How do bacteria resist antibiotics?

Bacteria also develop resistance to biocides. Traditional cleaning methods for equipment may have limitations. For example, patients who recieve a bed used by a prior patient with a bacterial infection are at an increased risk for being colonized and infected by that same bacteria. In addition, bacteria in a healthcare setting are exposed to disinfection which selects for the resistant members of that bacterial population. No-touch methods of disinfection, i.e. uv light or hydrogen peroxide vapor, of equipment may be an improvement. https://www.ncbi.nlm.nih.gov/pubmed/29214175# (Links to an external site.)Links to an external site..

8. Are biofilms a concern when disinfecting equipment? Why?

During the Flint, MI water crises as levels of chlorine fell in the drinking water cases of Legionnaires' disease increased. Legionnaires' disease is a water borne infectious disease caused by Legionella. One study reported that for every drop in cholorine concentration of mg/L of river water the odds of a reported case of Legionnaire's disease increased by 80%, thus establishing not causation but association. Some argued that Legionella is tolerant of chlorine, espceillay when it grows in biofilms and that the temperature difference between lake and river water accounts for the increase in Legionella infections. The recommended level of chlorine to treat water for drinking is 0.2-0.5 mg/L.

doi:10.1126/science.aat2210 Was Flintâs deadly Legionnairesâ epidemic caused by low chlorine levels in the water supply? David Shult (Links to an external site.)Links to an external site.z

Was Flintâs deadly Legionnairesâ epidemic caused by low chlorine levels in the water supply?

9. What effect would increasing the concentration of chlorine to 1.4 mg/L have had on the people who drink Flint water?

10. What happens to the water pipes when chlorine levels are increased to control bacteria? Could these uninteded consequences increase bacterial growth?

ochrecrocodile432

1. Discuss and decide upon characteristics that define each of the following broad groups

(domains, kingdoms) of organisms in order to attempt to define these major groups of life

on the planet. Although many characteristics may overlap from one group to the next,

attempt to define some traits that could be used to definitively (exclusively) place an

organism into a specific group. Finally, for each, what would the small subunit ribosomal

rRNA gene sequence tell us?

Viruses

Bacteria

Archaea

Protists (algae, protozoa)

Fungi

Animals

Plants

2. For the previous groups, which include representatives of ones that live as part of the

âhuman ecosystemâ? Provide some examples of each and where they can be found,

including both those that are part of our normal microbiota (i.e., not pathogens) and some

that are.

3. Considering the above and what weâve covered about these groups of organisms,

identify some structures/processes that hypothetically could be used to inhibit or kill

different types of pathogens without (hypothetically, at least) harming the human host.

4. Consider the microbial world. Which has a greater impact or importance â pathogens,

or benign microbes? Explain, giving examples.

5. A number of antibiotics target the bacterial 70S ribosome, exploiting structural

differences from the 80S ribosome found in humans and other eukaryotes. Although

effective at killing bacteria, these drugs can still have some toxicity to humans. In the

context of the endosymbiotic theory of evolution, provide a hypothesis as to why this

might be.

viridianeel412

True or False????? HELP

1. In the field of epidemiology, increased prevalence of a disease in a population is the universal signal that disease control and treatment are not effective.

2. Cytoplasmic membranes of prokaryotes typically contain more proteins than cytoplasmic membranes of eukaryotes microorganisms.
3. Sanger (or dideoxy) DNA sequencing produces relatively short DNA sequence data, but is by far the least expensive DNA sequencing method currently available.
4. The (italicizes) abbreviation lac Z refers explicitly to the beta galactosidase protein/ enzyme.
5. Archaeal pathogens have been linked to some of the most virulent infectious diseases. 6. A bacterial mutant that is unable to make an amino acid required for growth (relative to its wild-type strain is termed an auxotroph.
7. When counting plaques to enumerate phage particles (as in the BIOL2026 Laboratory), the plaque represents lacK, not presence, of bacterial growth.
8. Because of competition with plant roots for nutrients, microbial numbers in the rhizosphere are typically much lower than in surrounding bulk soil.
9. Chemolithoautotrophy is the strategy that facilitates the existence of biological communities deep beneath the earthâs surface and near hydrothermal vents.

10. In a primary treatment (1 Â°) system of a municipal sewage/ wastewater treatment plant, the main goal is to remove ammonium (NH4+) using specific bacterial enrichment cultures.
11. The small intestine in human is the main site of microbial breakdown of partially digested food.
12. Root nodules of legumes contain nitrogen fixing bacteria.
13. Anoxygenic photosynthesis involves using/ removing electrons from H2O.
14. Algae and Cyanobacteria utilize generally the same two-photosystem biochemical mechanisms in the light- reaction of photosynthesis.
15. In bacteria, the only known reason proton motive force is generated is to power ATP synthase in cellular respiration/ oxidative phosphorylation.
16. Anaerobic respiration is only carried out by prokaryotes, however fermentation can be carried out by both prokaryote and eukaryal microorganism.

17. The general structure and biochemical âmotorsâ of flagella are similar between Bacteria and eukaryotic cells, but fundamentally different in the Archaea.
18. Antibiotic resistance genes appeared in microbes (for the first time ever) only after human .. producing and releasing massive amounts of antibiotics to the environment.
19. Experiment by Esther Lederberg, and then later by Luria and Delbruck (that won them a Nobel prize), illustrated that genetic mutations in bacteria arose in the absence of selective pressures, rather than in response to selection.
20. Quaternary sensing is a mechanism used by bacteria to sense other bacteria and regulate gene expression accordingly (e.g. In the case of expressing the liac operon or initiating EPS production in biofilms).

MICB 201 Study Guide - Midterm Guide: Enol, Depolymerization, Ciprofloxacin

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