MICB 325 Study Guide - Quiz Guide: Pys, Intergenic Region, Consensus Sequence
Document Summary
1: let us return to cis-active sequences, using the cloned crt gene as an example. Recall that we roughly mapped sequences involved in the regulation of transcription initiation of the cloned crt gene, as shown on the right. 2: primer extension analysis yields three major bands, indicated by the arrows in the figure to the right [optional reading du et al 1999]: Du et al suggested a promoter with a -10 region just before the position of the major band, using the e. coli sigma70 consensus promoter sequence below as a model: See below for a representation of the dna sequence of this region, showing. Primer extension analysis of transcripts initiating from the crta- crti intergenic region. A and b show primer extension products initiated from the crti and crta promoters, respectively. The a, c, g, and t lanes represent dideoxynucleotide ladders generated with the same primers that were used for primer extension.
