BIO 1140 Study Guide - Midterm Guide: S Phase, Glycerol, Mitophagy
23 Jan 2018
School
Department
Course
Professor
Document Summary
How and when do we know what microscope is the best to use. Electron microscope the image is presented with light while the surroundings are black. Label and identify structures by tracing them down using the fluorescence that is on them. For example, you can put it on a microtubule or a transmembrane protein to see how they operate, move and generally function. has to be strong illuminated which may cause -damages delicate structures. The fluorescence isn"t permanent, so it start photobleaching after a while. During the preparation procedures. (antibody labelling must be labelled, treatment with detergent to permeabilize the membrane. ) Has to be stained before usage, which might damage or introduce artifacts. They aren"t living anymore so we wouldn"t be able to see the movement of the protein channels. They have a central vacuole organelle- turgor pressure. All of them have some sort of energy transforming units. Has many complex organelles to fulfill a cell"s function.
