BIO 1140 Study Guide - Final Guide: S Phase, Feulgen Stain, Flowering Plant
Document Summary
Most cells studied will be at the lower limits of visibility of light microscopes. Between the computer and the end of the counter. We will be well versed in bright field microscopy at the end of this lab. Optical microscopy technique (simplest) where you illuminate from below and observe from above. A system of lenses (objectives), a light source, geared mechanism for adjusting the distance between lens and object (working distance) The best image is not the largest it is the clearest. The ability to see two objects that are very close as two separate objects. The human eye has a resolving power of about 100um info, it is more comfortable and you can take pictures. resolution and wider fov. Revolving nosepiece = the different objectives are housed on the revolving nosepiece and as it rotates they are brought into the light path of the microscope.
