BIO 1140 Study Guide - Midterm Guide: Guanosine, Ribose, Active Transport
Document Summary
You have grown a culture of danio rerio gill cells and discovery that they are heavily contaminated with a mould, a type of fungus. To investigate the nature of a membrane protein, you treat an isolated membrane with the enzyme phospholipase c and then probe the membrane with a fluorescent marker for the protein of interest. By contrast, membranes probed with the fluorescent marker after changes in solution ph or ionic strength show a strong fluorescent signal. It is probably associated with the inner (cytosolic) leaflet of the lipid bilayer: the protein of interest is an integral protein, the protein of interest is linked to the membrane by a glycosylphosphatidylinositol (gpi) anchor. It is likely to be oriented towards the extracellular environment. Following transfer to an unknown solution, a human red blood cell rapidly swells and ultimately breaks. You are investigating the mechanism through which a solute (we"ll call it x), exits a plant cell across the plasma membrane.
