BIO 3153 Study Guide - Final Guide: Inverted Microscope, Micro Live, Microscope Slide
17 Jul 2014
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Document Summary
Isolated cells: inverted microscope light focused on specimen by condenser lenses objective and eye piece lenses focus an image in eye. Phase of light changes when light passes through a living cell light passing through a thick/dense part is retarted. Bright field: transmitted light, image obtained by simple light transmission through a cell in culture. Phase contrast: converts phase differences into changes in brightness, exploits wave properties, change when entering and exiting cell, exploits interference effects produced when 2 sets of waves recombine, exploiting shift creates contrast, observes phase shift through unstained cells. Differential interference contrast: similar to phase contrast but more definition, exploits interference effects, interferes with light passing through cell instead of exploiting phase shift, light is separated before interaction then put back together by prisms. Dark field: rays of light directed from side (lateral light source) so that only scattered light enters microscope lens/is shown, cell appears bright against dark background.
