BIM 4316 Study Guide - Midterm Guide: Bicinchoninic Acid Assay, Thermal Conductivity Detector, Molar Attenuation Coefficient
12 Jul 2018
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Biochemistry: covers the quantitative measurement of xenobiotics (drugs and their metabolites, and biological molecules in unnatural locations or concentrations) and biotics (marcomolecules, proteins, dna, large molecule drugs, metabolites) in biological systems. Sms cia crest(cid:499): size, mass, shape, charge, isoelectric point, affinity interactions, chemical reactivity, electron structure. Selenocysteine: amino acid that is present in several enzymes. Hypusine: a non-standard amino acid found in all eukaryotes and in some archaea, but not in bacteria. Measurement of adsorption of radiation: uv visible: the concentration of proteins can be determined by measuring the absorbance of ultraviolet radiation. Ir is useful for rapid on-line analysis of protein content: also requires a little sample prep and is non-destructive. Major disadvantage is the need for extensive calibration: nuclear magnetic resonance: nmr spectroscopy can be used to determine the total protein concentration of foods. Protein content is determined by measuring the area under the peak in an nmr chemical shift spectra that corresponds to the protein fraction.
